Publications on the project |
089 Methodology to assess the negative impact of carbon nanoparticles in the body and increase the carcinogenic risk |
Authors: | V.M. Mikhailenko | |
Summary: | Developed a methodology for assessment of the adverse impact of carbon nanoparticles on the organism. Conducted complex research of the carbon nanoparticles effect on normal and tumor cells, which included an assessment of the level of free radicals in the blood serum, liver cells and Erlich carcinoma cells in mice, the state of glutathione detoxification of xenobiotics, analysis of the level of damage and DNA repair. Changed parameters indicate the elevation of cancer risk, and can be used as a marker system for the analysis of the nanoparticles contribution to health risks increase. | |
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Edition: | Nanoscale systems and materials: studies in Ukraine. Kyiv : ID " Academperiodica " | | | 2014,
536-540,Russian |
089 The role of multiwall carbon nanotubes in the genome destabilization of 3T3 cells |
Authors: | Mikhailenko V.M., Kovaleva O.A., Stepanenko N.S.,
Makovetska L.I. | |
Summary: | The aim of this work was to study the cytogenetic changes in the cells of mouse embryonic 3T3 fibroblasts (clone 31), exposed to multiwall carbon nanotubes.
Materials and methods. 3T3 cells were treated with multiwall carbon nanotubes in two doses (100 and 400 mkg/ml) during 24 and 48 hours. Cytogenetic study involved an analysis of the mitotic regime and pathology of mitosis, fraction of cells with premature chromosome condensation, micronuclei, and nuclear protrusions. Metaphase plates were analyzed for modal class of chromosomes, polyploidy, the number of cells with chromosome aberrations, double minichromosomes and multiaberrant cells.
Results. Multiwall carbon nanotubes have a pronounced genotoxic effect on mouse embryonic 3T3 fibroblasts, which was accompanied by formation of micronuclei and various abnormalities of nuclear shape. High doses of multiwall carbon nanotubes caused inhibition of the proliferative activity on phase G2/M of cell cycle and accumulation of chromosome aberrations and multiaberrant cells. Multiwall carbon nanotubes caused the dose-dependent delay of cell division in prophase and metaphase during 48 hours. Registered cytogenetic changes indicate the formation of genomic disorders and genomic instability in the cells exposed to carbon nanoparticles.
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Keywords: | carbon nanoparticles, multiwall carbon nanotubes, micronuclei, сhromosome aberrations, genome destabilization | |
Edition: | Environment and Health | | | 2014,
19–23,Ukrainian |
089 Cytotoxicity of Carbon Nanotubes |
Authors: | Yu. Sementsov, T. Aleksyeyeva, I. Yermolenko [et al.] | |
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Edition: | Nanosystems, Nanomaterials, Nanotechnologies | | | 2013,
183-189,English |
089 Influence of carbon nanotubes (CNTs) on normal and tumor cells in vivo |
Authors: | T. Aleksyeyeva, L. Ieleiko, V. Mikhailenko | |
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Edition: | Nanosystems, Nanotechnologies, Nanomaterials | | | 2013,
43-46,Russian |
089 Carbon nanotubes filled polypropylene fibers : mechanical characteristics and biocompatibility |
Authors: | Yu.I. Sementsov, M.L. Pyatkovsky, G.P. Prikhod’ko [et al.] | |
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Edition: | Chemistry, physics and technology of surface | | | 2013,
191–195,English |
089 Influence of carbon nanotubes (CNTs) on bio adaptive properties of hydroxyapatite-CNT |
Authors: | Yu. Sementsov, T. Aleksyeyeva, N. Kartel [et al.] | |
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Edition: | Journal of Orthopedy, Traumatology and Prosthetic | | | 2013,
17-21,Russian |
089 Composites polypropylene-carbon nanotubes: structural features, physical and chemical properties and biocompatibility |
Authors: | Yu.I. Sementsov, M.L. Pyatkovsky, G.P. Prikhod’ko [et al.] | |
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Edition: | Physics, chemistry and technology of surface | | | 2013,
31-37,Ukrainian |
089 Determination of the toxic effects of nanotubes on hematopoietic stem cells and their progeny in vivo i in vitro |
Authors: | N.M. Bilko, L.O. Yeleyko-Hemych, N.K. Rodionova [et al.] | |
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Edition: | Modern problems of Toxicology | | | 2012,
43-44,Ukrainian |
089 Carbon nanoparticles impact on energy metabolism, genotoxicity and free radicals level |
Authors: | V. Mikhailenko, L. Ieleiko, A. Glavin, J. Sorochinska | |
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Edition: | Handbook of Free Radicals: Formation, Types and Effects | | | 2011,
523-538,English |
089 Biomarkers of nanoparticles impact on biological systems |
Authors: | V. Mikhailenko, L. Ieleiko, A. Glavin, J. Sorochinska | |
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Edition: | Nanomaterials: Risks and Benefits | | | 2010,
67-82,English |
The events in the framework of the project |
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089 5. Nanobiotechnology Purpose:To investigate the features of the carbon nanoparticles (СТP) interaction with biological systems and assess their impact on the free radical processes, changes of posttranslational modification and functional activity of some enzymes, energy metabolism, frequency and spectrum of cytogenetic damage and the development of genetic instability in the organism as the basis of markers for risks assessment due to interaction of nanoparticles with biological objects and estimation of their role in increasing of cancer risk. Expected results:Issue of new types of products: methods, theories Stage 1:Determination of CNP impact on the main parameters of energy metabolism, phospholipid profile and properties of cell plasmatic membranes. Stage 2:Investigation of СNP effect on free radical level of cells and the organism and their antioxidant enzyme activity. Determining the influence of CNP on apoptosis induction. Stage 3:Influence of multiwaled carbon nanotubes on activity and posttranslational modification of xanthine oxidoreductase enzyme, its effect on free radical processes, the content of glutathione, DNA damage and repair. Study of multiwaled carbon nanotubes on condition of glutathione detoxification system of xenobiotics. Stage 4:The influence of CNP on pool of polipotent hematopoietic stem cells in bone marrow of experimental animals. Study of CNP effect on nonspecific immune defense system of the organism. Stage 5:The study of cytogenetic effects of CNP on bone marrow cells and peripheral blood lymphocytes of animals. The study of genomic instability in terms of chromosomal aberrations in lymphocytes of the peripheral blood.
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